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Exongen A602 AccuProbe qPCR Master Mix User Manual
The AccuProbe qPCR Master Mix A602 is a 2× premix reagent designed for probe‑based real‑time PCR. It contains a chemically modified hot‑start DNA polymerase, UDG enzyme, dNTPs, dUTP, Mg and optional 50× ROX Reference Dye. The product is supplied in a 5 ml vial (A602‑01) and should be stored at –20 °C, protected from light, for up to two years.
The manual details how to set up a 20 µl reaction by adding template, primers, probe, optional ROX dye and water to achieve a 1× working concentration. Recommended PCR cycling steps, annealing/extension temperatures, and instrument‑specific ROX requirements are listed. Precautions include avoiding repeated freeze‑thaw cycles and mixing the premix by inversion rather than vortexing. This manual is written in Chinese.
Covers: Product Overview · Product Composition · PCR Conditions · Precautions · Recommended qPCR Instruments · ROX Reference Dye Usage
Latest Exongen manuals
Exongen A602 AccuProbe qPCR Master Mix User Manual
User Manual 3 pages · 9.3 MB · ZHThe Exongen A602 AccuProbe qPCR Master Mix User Manual provides composition, storage, recommended PCR conditions, and usage guidelines for researchers.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in Exongen manuals
How should the AccuProbe qPCR Master Mix be stored?
Store the reagent at –20 °C, protected from light, and it remains stable for up to two years.