Application note for automated IgG purification using PureProteome™ Protein G Magnetic Beads and KingFisher® Duo Particle Processor.
This document details an automated protocol for purifying IgG using PureProteome™ Protein G Magnetic Beads with the KingFisher® Duo Particle Processor. It compares automated and manual methods, showing comparable protein yield and purity. The protocol includes optimized parameters for mixing, binding, and washing steps. The document also provides ordering information for related magnetic beads and equipment.
The method uses 100 µL of bead slurry, PBS with 0.01% Tween® 20 as binding/wash buffer, and 0.2 M glycine HCl pH 2.5 for elution. Results from Bradford assay and SDS-PAGE confirm reproducibility and specificity. The beads are compatible with both manual and automated systems, including KingFisher® Duo and PureProteome™ Magnetic Stand.
| Binding Buffer | PBS with 0.01% Tween® 20 |
|---|---|
| Elution Buffer | 0.2 M glycine HCl, pH 2.5 |
| Bead Slurry Volume | 100 µL |
| Binding Time | 30 minutes |
Yes, the document evaluates and confirms the effectiveness of PureProteome™ Protein G Magnetic Beads for IgG purification using the KingFisher® Duo Particle Processor.
The elution buffer is 0.2 M glycine HCl, pH 2.5.
The automated method using KingFisher® Duo yields comparable protein yield and purity to the manual method.