EMD Millipore Manuals

EMD Millipore PureProteome™ Protein G Magnetic Beads with KingFisher® Duo Particle Processor

This document details an automated protocol for purifying IgG using PureProteome™ Protein G Magnetic Beads with the KingFisher® Duo Particle Processor. It compares automated and manual methods, showing comparable protein yield and purity. The protocol includes optimized parameters for mixing, binding, and washing steps. The document also provides ordering information for related magnetic beads and equipment.

The method uses 100 µL of bead slurry, PBS with 0.01% Tween® 20 as binding/wash buffer, and 0.2 M glycine HCl pH 2.5 for elution. Results from Bradford assay and SDS-PAGE confirm reproducibility and specificity. The beads are compatible with both manual and automated systems, including KingFisher® Duo and PureProteome™ Magnetic Stand.

Covers: Introduction · Antibody Purification─KingFisher® Duo Protocol · Materials and Methods · Results · Discussion · Ordering Information