User manual for Cytoskeleton's Cdc42 Activation Assay Biochem Kit, covering protocol, reconstitution, storage, and troubleshooting for measuring Cdc42…
This manual provides step-by-step instructions for performing the Cdc42 Activation Assay using the provided kit. It includes sections on background, kit contents, reconstitution procedures, storage conditions, assay protocols, and troubleshooting. The kit is designed for 20 pull-down assays and includes reagents such as PAK-PBD beads, anti-Cdc42 antibody, and control proteins. Users must supply cell lysates and standard Western blotting equipment.
| Kit Contents | PAK-PBD beads, Anti-Cdc42 monoclonal antibody, His-Cdc42 control protein, Cell Lysis Buffer, Wash Buffer, Loading Buffer, STOP Buffer, GTPγS stock, GDP stock, Protease Inhibitor Cocktail |
|---|---|
| Assay Capacity | Approximately 20 pull-down assays |
| Storage Conditions | Reconstituted components stored at 4°C or –70°C; lyophilized components stored desiccated at 4°C or –20°C |
PAK-PBD beads, His-Cdc42 control protein, GTPγS stock, and GDP stock should be snap-frozen and stored at –70°C. Antibody stored at 4°C. Protease Inhibitor Cocktail stored at –20°C.
This kit contains enough reagents for approximately 20 pull-down assays.
After reconstitution, the PAK-PBD beads should be snap frozen in liquid nitrogen and stored at –70°C.
The Anti-Cdc42 monoclonal antibody should be used at a 1:250 dilution.