Ampholytic-Magnetic-Submicron-Particles magnetic beads from Chongqing Boliangying enable pH‑controlled ion‑exchange extraction of nucleic acids, proteins…
The Ampholytic‑Magnetic‑Submicron‑Particles (MSP‑ZEW series) are sub‑micron magnetic beads (≈0.60 µm in vacuum, ≈1.0 µm in water) coated with a neutral polymer that encapsulates an Fe₃O₄ core. Their surface carries both positive and negative ionizable groups, allowing electrostatic attraction of nucleic acids at acidic pH and electrostatic repulsion for elution at basic pH. The beads are supplied as a neutral‑buffered suspension (20 mM PBS, pH 7.4, 0.09 % azide, 0.1 % antioxidant) and remain stable for at least 24 months when stored unopened, protected from light.
The document provides detailed reagent recipes, step‑by‑step extraction protocols for plasma cfDNA, microRNA and other biomolecules, and performance data such as a separation capacity of ~50 µmol g⁻¹ for Acid Red 13 at pH 4.4. It also includes stability tests, regeneration procedures, and comparative third‑party testing against competing magnetic bead products. This manual is written in Chinese.
| Particle size (vacuum) | ~0.60 µm |
|---|---|
| Storage solution | 20 mM PBS, pH 7.4, 0.09 % azide, 0.1 % antioxidant |
| Shelf life | ≥24 months unopened, stored 2~80 °C protected from light |
| Separation capacity (Acid Red 13, pH 4.4) | ~50 µmol g⁻¹ |
| pH operating range | 2.8 – 11.0 (stable), optimal adsorption at pH 3.0‑3.3, elution at pH 8.6‑8.9 |
The beads adsorb nucleic acids at acidic pH (≈3.0‑3.3) and elute them at basic pH (≈8.6‑8.9), and they remain stable from pH 2.8 to 11.0.
The MSP‑ZEW series beads tolerate pH ≈2.5 at room temperature for about 2 hours, but they demagnetize at 56 °C under the same pH.