Instruction manual for Chiralpak IA-3, IB-3, IC-3, ID-3, IE-3, and IF-3 columns covering reversed phase use, buffer preparation, and maintenance.
This manual provides guidance for using Chiralpak IA-3, IB-3, IC-3, ID-3, IE-3, and IF-3 chromatography columns in reversed phase mode and also describes how to switch to normal phase mode. It includes recommendations for flushing the column with miscible solvents, removing salts, and applying the regeneration procedure from the normal‑phase instruction sheet.
The document details mobile‑phase selection, buffer preparation for various pH ranges, and operational limits such as avoiding strong basic conditions and maintaining column temperature between 5 °C and 25 °C when pH exceeds 7. It also advises on the use of guard cartridges, organic‑modifier order, and precautions to prevent column clogging.
| Maximum pH for safe use | Avoid > pH 9 |
|---|---|
| Recommended temperature range at pH > 7 | 5 °C – 25 °C |
| Elution strength order of organic modifiers | CH3CN > EtOH > MeOH |
Flush the column carefully with a miscible solvent, remove any salts, and follow the regeneration procedure described in the normal‑phase instruction sheet.
Maintain the column temperature between 5 °C and 25 °C for maximum column life.
Acetonitrile (CH3CN) provides the highest elution strength, followed by ethanol and methanol.