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- EN
- Last added
- 2026-10-01
Featured Chiralpak manuals
Chiralpak IA-3, IB-3, IC-3, ID-3, IE-3, IF-3 Reversed Phase Instruction Manual
This manual provides guidance for using Chiralpak IA-3, IB-3, IC-3, ID-3, IE-3, and IF-3 chromatography columns in reversed phase mode and also describes how to switch to normal phase mode. It includes recommendations for flushing the column with miscible solvents, removing salts, and applying the regeneration procedure from the normal‑phase instruction sheet.
The document details mobile‑phase selection, buffer preparation for various pH ranges, and operational limits such as avoiding strong basic conditions and maintaining column temperature between 5 °C and 25 °C when pH exceeds 7. It also advises on the use of guard cartridges, organic‑modifier order, and precautions to prevent column clogging.
Covers: Switching between Reversed Phase and Normal Phase · Mobile Phase Selection · Buffer Preparation Examples · Column Temperature and pH Guidelines · Organic Modifier Elution Strength · Column Maintenance and Guard Cartridge Use
Latest Chiralpak manuals
Chiralpak IA-3, IB-3, IC-3, ID-3, IE-3, IF-3 Reversed Phase Instruction Manual
User Manual 2 pages · 154 KB · ENInstruction manual for Chiralpak IA-3, IB-3, IC-3, ID-3, IE-3, and IF-3 columns covering reversed phase use, buffer preparation, and maintenance.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in Chiralpak manuals
How do I switch the column from reversed phase to normal phase mode?
Flush the column carefully with a miscible solvent, remove any salts, and follow the regeneration procedure described in the normal‑phase instruction sheet.
From: Chiralpak IA-3, IB-3, IC-3, ID-3, IE-3, IF-3 Reversed Phase Instruction Manual
What temperature should be kept when using the columns at pH above 7?
Maintain the column temperature between 5 °C and 25 °C for maximum column life.
From: Chiralpak IA-3, IB-3, IC-3, ID-3, IE-3, IF-3 Reversed Phase Instruction Manual