The Bioland EasyPrepTM Total RNA Extraction Miniprep Plus Kit Manual provides step‑by‑step instructions, safety information, and troubleshooting for…
The EasyPrepTM Total RNA Extraction Miniprep Plus Kit offers a fast method to isolate up to 100 µg of total RNA from cultured cells or animal tissues in about 30 minutes. The kit includes RNA columns, DNA clearance columns, buffers, and optional RNA shredder columns for efficient homogenization and genomic DNA removal.
The manual covers storage and stability, safety precautions, detailed protocols for sample disruption, homogenization, RNA purification from both cultured cells and animal tissues, and a troubleshooting guide. It also lists kit contents, recommended reagent preparations, and related products.
| Maximum RNA yield per kit | 100 µg |
|---|---|
| Sample volume per RNA column | up to 700 µL |
| Component shelf life | 1 year from purchase |
| Ethanol concentration in RNA Wash Buffer | 80% (v/v) |
All kit components are guaranteed for one year from the date of purchase and can be stored at room temperature.
The kit can purify up to 100 µg of total RNA from eukaryotic cells or animal tissues.
Genomic DNA is removed by passing the lysate through DNA Clearance columns before binding RNA to the RNA column.