The TL-698 recombinant human B7-2/CD86 protein leaflet provides expression host, purity, molecular weight, storage, activity and reference information.
The TL-698 product is a recombinant human B7-2/CD86 protein expressed in human HEK293 cells. It consists of 221 amino acids, has a predicted molecular weight of 27.2 kDa, and is supplied as a lyophilized powder containing 6% mannitol in sterile PBS (pH 7.4). Purity is ≥95% as confirmed by SDS‑PAGE and HPLC, and endotoxin levels are below 0.1 EU/µg.
The protein is biologically active, binding human CTLA‑4 in a linear range of 20‑200 ng/mL. Lyophilized material can be stored at 4 °C for up to 24 months; once reconstituted, it should be kept at –20 °C for 6‑12 months and protected from repeated freeze‑thaw cycles. The leaflet also includes the mechanism of CD86 in immune activation and relevant literature references. This manual is written in Chinese.
| Expression host | Human HEK293 cells |
|---|---|
| Amino acid length | 221 aa |
| Molecular weight | 27.2 kDa (theoretical) |
| Endotoxin level | <0.1 EU/µg |
| Formulation | Lyophilized powder with 6% mannitol in sterile PBS (pH 7.4) |
| Lyophilized storage stability | Stable at 4 °C for 24 months |
| Reconstituted storage stability | Stable at –20 °C for 6‑12 months, avoid freeze‑thaw |
TL-698 is expressed in human HEK293 cells.
Lyophilized samples can be stored at 4 °C for up to 24 months; reconstituted solution should be kept at –20 °C for 6‑12 months and protected from repeated freeze‑thaw cycles.
The predicted theoretical molecular weight is 27.2 kDa.