User manual for Active Motif's MethylCollector™ MBD Capture Kit (version A1), detailing protocols for isolating methylated DNA for PCR analysis.
This document provides step-by-step protocols for using the MethylCollector™ MBD Capture Kit to isolate methylated DNA from cell or tissue samples. It includes instructions for DNA fragmentation, MBD2b protein binding, magnetic bead capture, washing, and elution. The kit supports downstream PCR analysis using provided primer mixes for genes like GAPDH, Xist, and NBR2. It is designed for research use only and includes controls for validating methylation status.
The manual also covers storage conditions for kit components, required additional materials, troubleshooting, and technical support information. It includes flowcharts, performance data, and real-time PCR analysis examples to guide users through the process.
| Sensitivity | 1 ng - 1 µg of fragmented genomic DNA |
|---|---|
| Kit Format | 30 reactions with excess reagents for 5 control reactions |
| Storage Temperature | Components stored at -80°C, -20°C, or 4°C as specified |
The kit can be performed on 1 ng - 1 µg of fragmented genomic DNA.
The kit includes control human, male genomic DNA digested with Mse I and PCR primer mixes for GAPDH, Xist, and NBR2.
Components are stored at -80°C, -20°C, or 4°C as specified in the table.