This manual provides instructions for the Active Motif CUT&Tag Assay Kit 53160, a reagent kit for studying histone modifications and transcription factors…
The Active Motif CUT&Tag Assay Kit 53160 is designed for studying histone modifications and transcription factors by revealing protein-DNA interactions. Unlike ChIP-Seq, this method uses antibody-targeted enzymes on fresh, unfixed cells to identify specific DNA binding sites with low background noise and no formaldehyde cross-linking artifacts. The kit supports generating 16 unique Illumina sequencing libraries from 50,000 to 500,000 cells per reaction. This manual is written in Chinese.
| Reactions per kit | 16 reactions |
|---|---|
| Compatible platform | Illumina |
| Minimum cells per experiment | 5,000 cells |
| ConA Beads capacity | 500,000 cells per 20 µL |
The minimum number of cells required for a single experiment is 5,000.
No, the kit does not require formaldehyde cross-linking, which eliminates false positive results caused by it.
The kit can generate 16 unique Illumina platform sequencing libraries.
The primary antibody should be incubated at 4°C for 2 hours or overnight.