Technical datasheet for the 4ABIO MDN3016-50 bone DNA column extraction kit, detailing components, storage and step‑by‑step protocol.
The MDN3016-50 kit from 4ABIO is designed for extracting DNA from animal bone or bone powder samples. It provides enough reagents for 50 micro‑extractions (approximately 300 mg of bone per run) and the whole procedure takes about 40–60 minutes.
The kit includes solutions A, B, C, a centrifuge adsorption column, wash buffers and elution buffer, together with a detailed protocol covering bone powder preparation, lysis, binding, washing, and DNA elution. Extracted DNA is suitable for PCR or real‑time PCR and can be stored at –20 °C for long‑term use. This manual is written in Chinese.
| Model | MDN3016-50 |
|---|---|
| Number of extractions | 50 |
| Solution A volume | 50 ml |
| Solution B volume | 15 ml |
| Solution C volume | 30 ml |
| Centrifuge adsorption columns | 50 sets |
| Universal wash buffer volume | 50 ml |
| DNA elution buffer volume | 10 ml |
| Storage temperature | 4 °C (room temperature transport allowed) |
One kit provides enough reagents for 50 micro‑extractions.
The whole procedure requires about 40 to 60 minutes.
Pre‑heating the DNA elution buffer to 60‑80 °C improves elution efficiency.
Yes, the DNA can be stored at –20 °C for long‑term preservation.