Instructions for using the 4ABio CME0006 mouse IL-6 ELISA kit to measure IL‑6 in serum, plasma or cell culture supernatants.
This manual describes the double‑antibody sandwich ELISA principle, kit components, and step‑by‑step procedures for detecting mouse IL‑6 in serum, plasma, or cell‑culture supernatants. It includes guidance on sample collection, reagent preparation, incubation conditions, washing steps, and result interpretation.
Key information covers storage conditions, handling precautions, assay sensitivity (detects IL‑6 < 4 pg/mL), specificity, and recommended dilution factors for samples and standards. This manual is written in Chinese.
| Assay Format | Double‑antibody sandwich ELISA |
|---|---|
| Sample Types | Mouse serum, plasma, or cell‑culture supernatant |
| Detection Limit | < 4 pg/mL |
| Specificity | Detects natural and recombinant mouse IL‑6; no cross‑reactivity with listed cytokines |
| Standard Curve Range | 0 – 500 pg/mL |
The kit can be used with mouse serum, plasma, or cell‑culture supernatant.
The assay can detect mouse IL‑6 concentrations lower than 4 pg/mL.
Incubate at 37 °C for 30 minutes after adding the enzyme‑conjugate working solution.