CHIRALPAK® AD-H chiral column user manual, introduces the structure, filler, retention time, mobile phase, column pressure, temperature and other aspects of use information.
This manual describes the CHIRALPAK AD‑H chiral column, including its dimensions (e.g., 150 × 4.6 mm ID, 250 × 4.6 mm ID, 250 × 10 mm ID, 250 × 20 mm ID) and recommended mobile phases such as n‑hexane/isopropanol (90:10) and a 50:50 methanol/ethanol mixture. It also lists flow‑rate limits (up to 1.5 ml/min) and pressure limits (not exceeding 100 Bar ≈ 1400 psi) with temperature up to 40 °C.
The document gives maintenance instructions, including the use of protective columns, recommended additives for acidic or basic samples, and storage procedures (replace mobile phase with n‑hexane/isopropanol 90:10 for storage longer than a week). It notes that the column can operate with 100 % methanol or ethanol, but after using such polar solvents only polar solvents should be used thereafter. This manual is written in Chinese.
| Column dimensions | 150 × 4.6 mm ID, 250 × 4.6 mm ID, 250 × 10 mm ID, 250 × 20 mm ID |
|---|---|
| Maximum pressure | 100 Bar (≈1400 psi) |
| Maximum temperature | 40 °C |
| Maximum flow rate | 1.5 ml/min |
| Recommended storage solvent | n‑hexane / isopropanol 90:10 (v/v) |
| Initial mobile‑phase recommendation | Methanol/ethanol 50:50 (v/v) |
| Pure polar solvent compatibility | Can use 100 % methanol or ethanol |
The column should not be operated above 100 Bar (approximately 1400 psi).
Replace the mobile phase with the storage solvent consisting of n‑hexane and isopropanol in a 90:10 ratio.
A 50:50 (v/v) mixture of methanol and ethanol is recommended as the initial mobile phase.
Yes, the column can be operated with 100 % methanol (or ethanol), but after using such polar solvents only polar solvents should be used thereafter.