The user manual provides storage conditions, composition, usage instructions, safety notes, and FAQs for Mei5 Biotechnology's M5 Protease Inhibitor Cocktail…
The M5 Protease Inhibitor Cocktail is a DMSO‑soluble mixture of six broad‑spectrum protease inhibitors designed to protect proteins during extraction, purification, and downstream assays such as Western blot, co‑IP, pull‑down, and immunofluorescence. It is intended for research use only and helps maintain protein integrity by inhibiting serine, cysteine, aspartic, and metalloproteases.
The product should be stored at –20 °C with a shelf life of two years. Before use, thaw to room temperature and add the cocktail to lysates at a 1:100 (v/v) ratio. Safety precautions include wearing a lab coat and disposable gloves, as the inhibitors are hazardous to skin, eyes, and respiratory tract. The manual also includes a detailed FAQ covering the rationale for using the cocktail, dilution guidelines, comparison with PMSF, and storage form recommendations. This manual is written in Chinese.
| Storage temperature | -20 °C |
|---|---|
| Shelf life | 2 years |
| AEBSF concentration | 104 mM |
| Aprotinin concentration | 80 µM |
| Bestatin concentration | 5 mM |
| E‑64 concentration | 1.5 mM |
| Leupeptin concentration | 2 mM |
| Pepstatin A concentration | 1.5 mM |
The cocktail provides low‑toxicity, broad‑spectrum protection that maximally preserves proteins from degradation, which is especially important for low‑abundance samples.
Yes, the cocktail should be diluted according to the recommended 1:100 (v/v) ratio; proper dilution is essential for effective inhibition.