A rapid detection method for novel coronavirus nucleic acid based on real-time fluorescent RT-PCR
The present invention provides a rapid detection method for novel coronavirus nucleic acid, which simultaneously detects three important regions of novel coronavirus genes, ORF1ab, E gene and N gene, in one PCR reaction tube, with low detection cost, high flux, high sensitivity and good accuracy. The present invention is simple to operate, and only needs to perform real-time fluorescent RT‑PCR amplification detection on the RNA extracted from the sample. The present invention has a fast detection speed, and the whole detection process takes only 60 minutes, meeting the clinical rapid detection needs.
