One-step dual CRISPR/Cas9 guide RNA cloning protocol

We describe a novel CRISPR/Cas9 dual guide RNA cloning protocol. This protocol is rapid, inexpensive and allows re-usage of gRNA oligonucleotides. The protocol is applicable to cloning gRNAs into virtually any CRISPR/Cas9 backbone that allows cloning by Golden Gate.

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Published
16 November, 2023
Updated
16 November, 2023
MD5 checksum
78E2FDF7E4DD3876BDB30997A2133BBE
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One-step dual CRISPR/Cas9 guide RNA cloning protocol PDF
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