A manual about EnVisionTM FLEX Systems
This manual provides a detailed protocol for operating the EnVision FLEX Systems. It includes step-by-step instructions for rinsing, applying primary antibodies, using peroxidase-blocking reagents, and staining with substrate solutions. Reagent preparation guidelines are also included, such as dilution ratios for wash buffer and substrate working solutions. The protocol covers both standard and FLEX+ system variations, with identical steps for reagent preparation and storage conditions.
| Wash Buffer Dilution | 1:20 |
|---|---|
| Substrate Working Solution | 1 mL EnVision™ FLEX Substrate Buffer + 1 drop EnVision™ FLEX DAB+ Chromogen |
| Storage Condition | Dark and cold (2 °C – 8 °C) |
| Use Within | 5 days |
Dilute 1 part 20x buffer to 20 parts water (e.g., 50 mL buffer + 950 mL water for 1 L).
Store unused reagents in the dark at 2 °C to 8 °C.
It can be used within 5 days.