This document is a user guide which provides instructions on how to configure GeneBLAzer® Assay on Molecular Devices SpectraMax® M5/M5e Microplate Reader.
The document provides step‑by‑step instructions for setting up the SpectraMax M5/M5e microplate reader to run Life Technologies GeneBLAzer® assays. It includes recommended optics wavelengths, software configuration, plate type selection, and data analysis settings, and it is also applicable to compatible SpectraMax models.
Users are instructed to open SoftMax® Pro 6, select the GeneBLAzer protocol, configure optics (excitation 410 nm, emissions 460 nm and 530 nm), set the plate format (96 or 384 wells), enable bottom‑read mode, use automatic PMT gain, and run the read. After acquisition, the guide shows how to assign wells to template groups and view results for further analysis.
| Excitation wavelength | 410/9 nm |
|---|---|
| Emission 1 wavelength | 460/15 nm |
| Emission 2 wavelength | 530/15 nm |
| Emission 1 cutoff filter | 435 nm |
| Emission 2 cutoff filter | 515 nm |
| PMT gain setting | Automatic |
| Supported plate formats | 96 or 384 wells |
The setup uses SoftMax® Pro 6 software.
Set the PMT gain to "Automatic" to cover the full range of sample brightness.
Both 96‑well and 384‑well plate formats can be selected.