This article introduces the basic working principle, equipment overview, hardware operation instructions, software usage instructions, safety precautions, and daily cleaning and maintenance of the LSM 700 laser scanning confocal microscope.
This document serves as a concise guide for users new to the Zeiss LSM 700 confocal laser scanning microscope. It explains the fundamental working principles of the device, including optical sectioning and signal detection via PMT and Pinhole systems.
The manual provides step-by-step instructions for hardware operation, such as startup/shutdown sequences using Axio Observer Z1 or Z2 microscopes, and detailed software usage for 2D/3D scanning, spectral splitting, and image processing. It also includes safety precautions regarding laser use and daily maintenance procedures. This manual is written in Chinese.
The Zeiss LSM 700 is a laser confocal scanning microscope system used for optical sectioning and three-dimensional reconstruction of samples.
The system can connect up to four laser lines with selectable wavelengths including 405/445 nm, 488 nm, 555 nm, and 639 nm.
The variable secondary dichroic mirror has a continuously adjustable splitting range from 420 to 630 nm.