The Zeiss LSM 880 with Airyscan User Manual provides safety instructions, equipment specifications, and step‑by‑step procedures for operating the inverted…
The Zeiss LSM 880 with Airyscan is an inverted confocal laser scanning microscope equipped with a 32‑channel GaAsP Airyscan detector that improves spatial resolution and signal‑to‑noise ratio. The guide describes the instrument’s hardware, laser lines, detectors, and optical components, and explains how the Airyscan technology achieves a 1.7‑fold resolution gain.
The manual covers safety rules for laser use, detailed equipment specifications, startup and shutdown procedures, sample preparation, imaging acquisition modes (including Z‑stack, time series, tile scan, and multidimensional acquisition), and advanced techniques such as FRAP, FRET, and FLIM. It also provides guidance on using the Zen software interface and configuring the microscope for various experimental needs.
| Microscope stand | Axio Observer 7 SP Inverted Microscope Stand |
|---|---|
| Motorized stage | 130 x 100 STEP |
| Laser line (405 nm) | Diode, max power 30 mW |
| Airyscan detector | 32‑element GaAsP PMT array |
Never look directly into the laser beam or its reflections, never interfere with the beam path, and always keep the interlocks engaged; wear gloves when handling toxic lens cleaning solution and follow the listed first‑aid procedures.
The Airyscan detector provides a 1.7‑fold resolution gain in all spatial directions compared with conventional confocal detection.