This application report describes the results of using an Agilent 1100 Series Capillary Electrophoresis System and Agilent 1100 MSD Mass Spectrometer to analyze the digestion of bovine serum albumin (BSA).
This document presents application notes for analyzing digested Bovine Serum Albumin (BSA) using an Agilent 1100 Capillary HPLC system coupled with an Agilent 1100 MSD Trap. It details the specific instrument settings, including the Zorbax SB C18 column, mobile phase composition, and gradient program used to generate the chromatogram shown in Figure 1.
The document also describes the mass spectrometry analysis, presenting the MS/MS spectrum of a specific peak and the subsequent protein identification process using the MASCOT search engine. It includes details on the database search parameters and the resulting identification of the protein precursor.
| Mass range | 350 ~ 2000 ( m/z) |
|---|---|
| Column | Zorbax SB C18 (3.51 1m, 0.3x 150mm ) |
| Ionization | ESI |
| Nebulizer | Nz (40 psi) |
| Drying gas | N2¢8 L/min, 300 °C |
| Polarity | Positive |
| Mode | Auto MS/MS |
| Auto MS/MS range | 400 ~ 800¢( m/z) |
| Oven temp. | 40°C |
The sample analyzed was digested BSA (Albumin Bovine Reduced Carboxy-methylated).
The software used for the search was MASCOT.
The identified protein was ALU BO¥IN SERUM ALBUMIN PRECURSOR - BOS TAURUS (BOVINE).