This document describes a method for analyzing the digestion products of bovine serum albumin using enzymatic digestion and ion trap mass spectrometry. The analysis was performed using Agilent 1100 Capillary system and MSD Trap mass spectrometer, and the data were searched in the MASCOT database. Based on the Mass Spectrum and Deconvoluted results, the digestion products of BSA were successfully identified.
This document presents an application note titled LC/MS Application News No.97, which details the analysis of digested Bovine Serum Albumin (BSA). The study utilizes an Agilent 1100 Capillary LC system coupled with an Agilent 1100 MSD Trap mass spectrometer.
| Instrument | Agilent 1100 Capillary system |
|---|---|
| Mass range | 350 ~ 2000 ( m/z) |
| Ionization | ESI |
| Column | Zorbax SB C18 (3.51 1m, 0.3x 150mm ) |
| Mobile phase A | 0.05% TFA |
| Mobile phase B | CH3CN (0.05% TFA ) |
| Oven temp. | 40°C |
| Injection vol. | 0.01 µL |
The sample used was digested BSA (Albumin Bovine Reduced Carboxy-methylated).
The software used for the analysis was MASCOT.