This note describes the combination of an Agilent 8453 spectropho- tometer and Pro-K.2000 rapid kinetics system (Applied Photophysics Ltd., Leatherhead, UK), which provides a complete solution for kinetics measurements of complex chemical reactions.
This document describes a solution for measuring reaction kinetics in chemical and biochemical sciences by combining an Agilent 8453 spectrophotometer with the Pro-K.2000 rapid kinetics system from Applied Photophysics Ltd. The system addresses technical challenges such as the controlled initiation of reactions and reliable triggering of data acquisition.
The Pro-K.2000 system comprises a stopped-flow mixing device and kinetic analysis software. The hardware includes a stopped-flow mixing accessory with pneumatic drive that rapidly mixes two reagents and triggers the spectrophotometer. The software provides analysis and reaction simulation facilities, allowing for singular value decomposition (SVD), global fitting, and visualization of kinetic data.
The observation cell provides absorbance light path lengths of 2 and 10 mm.
The design allows volumes of less than 100 pl per sample to be mixed efficiently.