This document introduces the design features of the Agilent Cary 50 and Cary 60 UV-Vis spectrophotometers, including double-beam design and internal reference detector.
This document provides a technical overview of the Agilent Cary 60 UV-Vis spectrophotometer, highlighting its dual-beam design with two detectors and internal reference. It explains how the instrument compensates for source intensity fluctuations and solvent/cuvette effects without requiring a reference cuvette.
The guide details the step-by-step procedure for obtaining baseline-corrected spectra using the WinUV software. It includes instructions on performing a zero measurement (blank) and measuring the sample, along with the mathematical equations used by the software to calculate corrected absorbance values.
The Cary 60 can measure turbid solutions up to 3.3 Abs accurately because the beam is focused near the sample detector.