This is a document about the method of determining the content of melamine, ammeline, ammelide and cyanuric acid in infant milk-based formula and other food and feed products using the Varian 220-MS Ion Trap GC/MS/MS.
The document details an analytical method developed to detect and quantify melamine and related compounds (cyanuric acid, ammeline, and ammelide) in various matrices, including infant milk-based formula, pet food, and other feed products. The method addresses the issue of kidney failure caused by these contaminants.
The procedure utilizes a Varian 220-MS ion trap mass spectrometer coupled with a gas chromatograph (431-GC). It involves sample extraction using diethylamine, water, and acetonitrile, followed by trimethylsilyl (TMS) derivatization. The analysis is performed in MS/MS mode to eliminate matrix interference and achieve quantitative results down to 0.4 µg/g.
| Quantitative range | 20 to 2000 ppb (0.4 to 40 µg/g) |
|---|---|
| Column | Varian FactorFour VF-Xms capillary column, 30 m x 0.25 mm x 0.25 um |
| Carrier Gas Flow | Helium at 1 mL/min |
| Oven Program | 75 °C for 1 min to 300 °C at 15 °C/min, and hold 4 min |
The method can quantitate melamine and related compounds at a concentration as low as 0.4 µg/g.
In MS/MS mode, interference from the matrix is eliminated, providing extra confidence for identification.