This application note describes the use of Agilent PL aquagel-OH columns for the aqueous size exclusion chromatography analysis of polyvinyl alcohols. These columns combine high pore volume and high column efficiency (>35,000 plates/meter) for maximum resolution. The calculated molecular weight averages were compared with manufacturers’ quoted viscosity values. Calibration was done using pullulan polysaccharides.
This document presents an application note by Greg Saunders and Ben MacCreath from Agilent Technologies, Inc. It details the analysis of three polyvinyl alcohol samples using aqueous Size Exclusion Chromatography (SEC). The study utilizes Agilent PL aquagel-OH columns, which combine high pore volume and high column efficiency for maximum resolution. Calibration was performed using pullulan polysaccharides to compare calculated molecular weight averages with manufacturers' quoted viscosity values.
| Column Part Number | PL1149-6840 |
|---|---|
| Column Dimensions | 300 x 7.5 mm |
| Flow Rate | 1.0 mL/min |
| Detection Method | RI |
| Eluent Composition | 0.2 M NaNO3 + 0.01 M NaH2PO4 |
The analysis used 2x PL aquagel-OH 40 8 pm columns.
The flow rate was set to 1.0 mL/min.