Large volume injection (LVI) methods can improve the sensitivity and selectivity of existing analytical methods. This tutorial introduces the LVI capabilities of the Agilent Multimode Injector (MMI), including Hot Splitless Injection, Cold Splitless Injection, and Solvent Vent.
This document is a tutorial for the Agilent Multimode Injector (MMI), focusing on Large Volume Injection (LVI) techniques to improve trace analysis. It explains the benefits of LVI, such as increased sensitivity and reduced sample consumption, and details the challenges associated with traditional hot splitless injection, including analyte degradation and needle fractionation. The text introduces the MMI's programmable temperature capabilities as a solution to these issues. This manual is written in French.
| Sample Volume Range | a few microliters to at least one milliliter |
|---|---|
| Injector Temperature (Hot Splitless) | 250 °C |
| Column Flow Rate | 4ml/min |
| Syringe Capacity | 10 pl |
LVI improves trace analysis by increasing the mass of the analyte reaching the detector, leading to higher signal-to-noise ratios and lower detection limits.
Large Volume Injection can be applied to volumes ranging from a few microlitres up to at least one millilitre.
Most standard injector liners have a nominal volume of one millilitre.