This article introduces the large volume injection (LVI) technique of Agilent Technologies' multimodal injector (MMI), including hot splitless mode, cold splitless mode, and solvent discharge. Increasing the injection volume can improve residue analysis. The technique also allows for a reduction in the initially processed sample amount.
The document describes a practical exercise on the Agilent Multimode Injector (MMI) designed to demonstrate Large Volume Injection (LVI) techniques. It explains the benefits of LVI, such as improved sensitivity for trace analysis and reduced solvent consumption, compared to traditional injection methods.
The guide details two specific operational modes: hot splitless injection and cold splitless injection. It outlines the procedural steps for setting up the instrument, including column specifications (HP-5), injector temperatures, flow rates, and detector settings using a Flame Ionization Detector (FID) with a standard test sample. This manual is written in Italian.
The technique can be applied to injection volumes ranging from a few microliters up to one or more milliliters.
The injector temperature should be set to 250 °C for the hot splitless injection method.
The FID MDL test sample used in the exercise has the product code 5188-5372.