This document is a guide for packing PLRP-S material into the laboratory Load & Lock™ columns. It includes packing pressure, operational instructions and personnel.
The document outlines safety guidelines and preparation steps for personnel with knowledge of Dynamic Axial Compression (DAC) column packing. It details how to prepare a slurry from dry PLRP-S powder using acetonitrile:water solvent, including specific concentrations and mixing methods like shaking or ultrasonication.
A piston packing pressure of approximately 650 psi (45 bar) is recommended.
The column must be allowed to stand or equilibrate for 10 minutes after packing.