PfuUltra II Hotstart PCR Master Mix is a PCR amplification kit produced by Agilent Technologies, which contains 2×PfuUltra II Fusion HS DNA polymerase, optimized PCR reaction buffer, magnesium ions and dNTPs.
This document provides product details for the PfuUltra II Hotstart PCR Master Mix, available in two catalog sizes: 100 reactions (#600850) and 400 reactions (#600852). The mix contains PfuUltra TI Fusion HS DNA polymerase, an optimized buffer, magnesium, and dNTPs. It is designed for high accuracy, specificity, and long target-length capability.
| Storage Temperature (Unthawed) | -20°C |
|---|---|
| Storage Temperature (Thawed) | 4°C |
| Guaranteed Activity Period | 6 months |
| Final Mg Concentration (1X) | 2 mM |
For genomic DNA, use 15 seconds; for cDNA, use 30 seconds.
Generally, 100 ng of genomic DNA template is recommended.