Agilent PfuUltra High-Fidelity DNA Polymerase Instruction Manual

About this manual

The document serves as an instruction manual for the PfuUltra High-Fidelity DNA Polymerase enzyme system. It details the materials provided with different catalog numbers, storage conditions at -20°C, and specific ordering information for Agilent Technologies Stratagene Products Division.

A significant portion of the text is dedicated to critical optimization parameters for PCR reactions using this enzyme. Key topics include extension times based on target length (1 min/kb for targets <6 kb, 2 min/kb for larger targets), enzyme concentration recommendations (2.5 U vs 5.0 U per reaction), primer design guidelines, and the impact of dUTP accumulation on PCR efficiency.

What's inside

• MATERIALS PROVIDED
• STORAGE CONDITIONS
• CRITICAL OPTIMIZATION PARAMETERS
• EXTENSION TIME
• ENZYME CONCENTRATION
• PRIMER-TEMPLATE PURITY AND CONCENTRATION
• PRIMER DESIGN
• DEOXYNUCLEOSIDE TRIPHOSPHATES
• TROUBLESHOOTING
• REFERENCES

Specifications

Frequently asked questions

What is the recommended extension time for targets greater than 6 kb?

Use an extension time of 2.0 minutes per kb.

What is the suggested final concentration for primers?

Use a final concentration of 0.2–0.5 μM.

What storage condition is required for all components?

All components must be stored at –20°C.