Agilent pET System Vectors Instruction Manual

pET System Vectors and Hosts INSTRUCTION MANUAL, includes LIMITED PRODUCT WARRANTY, ORDERING INFORMATION AND TECHNICAL SERVICES


Agilent pET System Vectors Instruction Manual - cover page
Brand
pET
Document type
User Manual
Language
English
Pages
26
File format
PDF
File size
232 KB
Published
06 June, 2012
Updated
30 September, 2023
MD5 checksum
D05088500792A651EF0F5CBD6DDB17AF

About this manual

This document serves as an instruction manual for the Agilent pET System Vectors and Hosts kit. It details the materials provided in the kit, including specific vector series (pET-3 and pET-11) and competent cell strains like BL21-Gold(DE3). The guide explains that these vectors are designed for cloning and expressing recombinant proteins in E. coli using the T7 expression system.

What's inside

  • Materials Provided
  • Storage Conditions
  • Academic and Nonprofit Laboratory Assurance Letter
  • Introduction to pET Expression Vectors
  • Cloning Protocol
  • Transformation Protocol
  • Expression Protocols (IPTG Induction)
  • Induction by Infection with Lambda CEG
  • Troubleshooting

Specifications

Vector Storage Temperature-20°C
Competent Cells Storage Temperature-80°C
Control Plasmid Concentration0.1 ng/ul in TE buffer

Frequently asked questions

What is the intended use of this product?

The product is for in vitro use only.

Can these materials be used for commercial purposes?

No, a separate license is required for any commercial use.

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