This manual introduces the use of AffinityScript One-Step RT-PCR Kit, including materials, use conditions, operation steps, etc.
The document provides instructions for the Agilent AffinityScript One-Step RT-PCR Kit, which includes Herculase II RT-PCR 2x Master Mix and AffinityScript RT/RNase Block. The kit is designed for fast, high-yield, reliable single-tube RT-PCR applications suitable for gene profiling.
| Reactions supported | 100 RT-PCR reactions |
|---|---|
| RT temperature range | 42°C to 55°C |
| Optimal RNA OD ratio | 1.8—2.0 |
| RT inactivation temperature | 92°C for 1 minute |
RNase-free water, a temperature cycler, thin-walled PCR tubes or plates, and RT-PCR primers.
Full activity is guaranteed for one month once the master mix has been thawed.