SureStart Taq DNA Polymerase Instruction Manual
Agilent SureStart Taq DNA polymerase is a modified version of Taq2000 DNA polymerase with hot start capability designed to improve PCR amplification reactions by decreasing background and increasing the amplification of desired products. The enzyme remains inactive until stringent temperatures are reached, allowing for reaction setup at ambient temperatures without nonspecific primer annealing.
| Product Type | SureStart Taq DNA polymerase (5 U/μl) |
|---|---|
| Storage Temperature | -20°C |
| Recommended Primer Concentration | 0.1–0.5 μM |
| Optimal Enzyme Concentration (<2 kb) | 1.25 U/50-μl reaction |
| Denaturation Temperature Range | 92–95°C |
| Extension Temperature | 72°C |
No, this product is for research use only and not for use in diagnostic procedures.
It can be activated by adding an initial step of 9–12 minutes at 92–95°C or slowly during temperature cycling.
Melting temperatures between 55° and 80°C generally yield the best results.