This document is the user manual for Agilent ABLE C Strain and ABLE K Strain Excision Protocol
This document provides an instruction manual for the Agilent ABLE C strain and ABLE K strain. These E. coli strains are designed to reduce the copy number of common cloning vectors by 4- to 10-fold compared to standard hosts like XL1-Blue, thereby enhancing the probability that toxic clones will be propagated without killing the host cells.
| Copy number reduction (ABLE C vs XL1-Blue) | ~4-fold |
|---|---|
| Copy number reduction (ABLE K vs XL1-Blue) | ~10-fold |
| Bacterial Glycerol Stock Storage Temperature | -80°C |
| ExAssist helper phage concentration | >1 x 10^9 pfu/ml |
| ABLE C strain antibiotic resistance | Kanamycin, Tetracycline |
Both strains have the genotype E. coli C lac(LacZ@ ) [Kan' McrA McrCB° McrF Mrr HsdR(i¢ my }] [F’ proAB lacl#ZAM15 Tn 10 (Tet’].
Bacterial glycerol stocks should be stored at -80°C.
No, the ExAssist helper phage cannot replicate in SOLR or ABLE strains because it contains an amber mutation that prevents replication in nonsuppressing E. coli strains.