This document provides instructions for using Brilliant II QPCR Master Mix with ROX, including the features and characteristics of different models and packaging options.
This document provides instructions for the Agilent Brilliant II QPCR Master Mix with ROX (Catalog #600805-#600817), available in High ROX (500 nM) and Low ROX (30 nM) formulations. The master mix includes SureStart Taq DNA polymerase, nucleotides, and an optimized buffer for quantitative amplification of genomic DNA, plasmid DNA, and cDNA.
| High ROX concentration | 500 nM |
|---|---|
| Low ROX concentration | 30 nM |
| Reaction volume capacity | 400 reactions (25-μl) |
Store the 2× master mix at 4°C after thawing. Once thawed, full activity is guaranteed for 6 months.
All components must be stored at –20°C when they are first received.
Full activity is guaranteed for 6 months once the product has been thawed.
The excitation wavelength is 584 nm and the emission wavelength is 612 nm.