This manual describes the operation of the Gene Pulser Xcell electroporation system, including how to connect the instrument, prepare the electroporation vector, set parameters, and perform electroporation.
This manual covers the Gene Pulser Xcell™ electroporation system, a modular pulse generator used for bacterial, fungal, and mammalian cell transformation. It includes safety information, unpacking, installation of the main unit, CE and PC modules, and the ShockPod, as well as detailed operating instructions for exponential decay and square‑wave pulses.
The document also presents electroporation theory, factors affecting efficiency, step‑by‑step protocols for various cell types, calibration procedures, and a full specification table. Warranty terms, regulatory compliance (EN61010, EN61326), and troubleshooting guidance are provided to ensure safe and reliable use in the laboratory.
| Voltage range | 10–3,000 V |
|---|---|
| Capacitance range | 10–500 V, 25–3,275 µF in 25 µF increments |
| Parallel resistance range | 50–1,000 Ω in 50 Ω increments plus infinity |
| Minimum sample resistance | 20 Ω at 10–2,500 V; 600 Ω at 2,500–3,000 V |
| Operating temperature | 0–35 °C |
| Maximum power consumption | 240 W (during short charging periods) |
The system is warranted for one year against defects in materials and workmanship, with exclusions for improper operation, unauthorized repairs, and certain parts.
It meets the EN61010 electrical safety standard and the EN61326 EMC requirements for Class A laboratory equipment.
The high‑voltage circuit can generate pulses up to 3,000 V, while the low‑voltage circuit is limited to 500 V.