Rat total protein (TP) enzyme-linked immunosorbent assay (ELISA) kit instruction manual, which is mainly used to measure the total protein (TP) content in rat serum, plasma or related tissue fluid.
This manual describes the principle, reagents, sample requirements, and step‑by‑step procedure for the Da Ding rat total protein (TP) ELISA kit. The assay uses a double‑antibody sandwich format with HRP‑labeled detection antibody and TMB substrate, and results are read at 450 nm.
The document includes detailed instructions for reagent preparation, plate washing, incubation, color development, termination, and data calculation, as well as safety and storage guidelines. This manual is written in Chinese.
| Detection range | 20 µg/ml – 500 µg/ml |
|---|---|
| Plate format | 96‑well plate |
| Shelf life | 6 months |
Serum, plasma, brain fluid, and peritoneal fluid can be measured directly.
The assay can detect rat total protein concentrations from 20 µg/ml up to 500 µg/ml.
No, samples containing NaN₃ should not be used because NaN₃ inhibits HRP activity.