This kit is used to measure the content of cholesterol (CH) in rat serum, plasma, cell supernatant and related liquid samples.
The kit is designed to determine cholesterol (CH) levels in rat serum, plasma, urine, cell culture supernatants and tissue extracts using a double‑antibody sandwich ELISA. Purified anti‑cholesterol antibodies coat the micro‑plate wells, and HRP‑labeled antibodies form a complex that produces a color change measured at 450 nm.
The document details kit components, storage conditions (2‑8 °C, 6‑month validity), sample handling, step‑by‑step assay instructions (standard preparation, sample dilution, incubation, washing, substrate addition, stopping reaction), and how to calculate concentrations from the standard curve. Performance specifications such as detection range and linearity are also provided. This manual is written in Chinese.
| Well Configuration | 48‑well or 96‑well |
|---|---|
| Detection Range | 1 pmol/L – 20 pmol/L |
| Linearity (R²) | ≥ 0.990 |
| Shelf Life | 6 months |
| Standard Concentration | 27 pmol/L (0.5 mL per bottle) |
Serum, plasma, urine, cell culture supernatant, and tissue homogenates can be measured.