This assay kit uses a double antibody sandwich assay to detect SARS virus N protein in human or animal serum, and directly judges whether the subject is infected with SARS virus by detecting the presence or absence of N protein.
This manual provides an overview of the SARS N protein ELISA test kit, including the biological role of the N nucleocapsid protein and the double‑antibody sandwich principle used for detection. It outlines the intended application for laboratory testing of human or animal serum samples.
The document details the kit composition, reagent preparation, incubation steps, detection wavelength, and storage conditions, along with precautions to ensure reliable results. This manual is written in Chinese.
| Incubation temperature | 37 °C |
|---|---|
| Incubation time | 1 hour |
| Detection wavelength | 430 nm |
| Standard concentration range | 0–50 ng/ml |
The kit uses a double‑antibody sandwich ELISA to detect SARS N protein in human or animal serum.
Standard protein is diluted to a series of concentrations, OD values are measured at 430 nm, and the curve is plotted with CurveExpert or similar software.
Store the kit at 2–8 °C; it remains valid for six months.