This document describes the instructions for using the Turtle Progesterone (P) Enzyme-Linked Immunosorbent Assay (ELISA) Kit, as well as the composition of the kit and the operating steps. The kit is used to measure the progesterone (P) content in turtle serum, plasma, and related liquid samples, using a double antibody sandwich method. The kit has the characteristics of high sensitivity and easy operation.
The document describes the components of the ELISA kit, how to prepare standards and samples, and the detailed assay workflow. It covers reagent mixing, incubation steps, washing, color development with TMB, and measurement of absorbance.
Additional sections give important precautions, storage conditions, and guidance on interpreting results, ensuring reliable and reproducible hormone quantification. This manual is written in Chinese.
| Detection range | 3 µg/L – 80 µg/L |
|---|---|
| Incubation temperature and time | 37 °C for 30 min |
| Measurement wavelength | 450 nm |
The kit can detect progesterone concentrations from 3 µg/L up to 80 µg/L.
Incubate the sealed plate at 37 °C for 30 minutes.
Absorbance is measured at 450 nm.