The rat leptin (LEP) chemiluminescent assay kit is used for quantitative determination of leptin (LEP) in rat serum, plasma, tissue homogenates, cell culture supernatants or other related biological fluids by chemiluminescent assay.
This manual provides detailed procedures for the USCNLIFE rat LEP chemiluminescence kit, covering reagent preparation, sample collection, assay steps, and plate washing. It also includes guidance on storage conditions for samples and reagents to ensure reliable results.
Key sections describe the preparation of standard solutions, the step‑by‑step assay workflow, calculation of LEP concentrations, and important notes on specificity, handling, and troubleshooting. This manual is written in Chinese.
| Detection range | 78 pg/mL – 5000 pg/mL |
|---|---|
| Standard concentrations | 2500, 1230, 623, 312, 136, 78 pg/mL |
Serum should be centrifuged at 1000 × g for 20 min, the supernatant collected, and then stored at –20 °C or –80 °C.
Dilute the 5000 pg/mL stock solution with the provided diluent to obtain standards of 2500, 1230, 623, 312, 136, and 78 pg/mL.
Only reagents supplied by USCNLIFE should be used to ensure accurate results.