BP180 antibody detection kit (ELISA) is an in vitro diagnostic reagent kit, mainly used to detect BP180 antibodies in human serum, which can be used as an auxiliary diagnostic method for diagnosing bullous pemphigoid.
The BP180 Antibody ELISA Test Kit (model 7695E) is designed for qualitative detection of BP180 antibodies in human serum using an enzyme‑linked immunosorbent assay. The kit employs micro‑well strips coated with BP180 antigen and a colorimetric substrate to generate measurable absorbance at 450 nm.
The kit includes 48 wells, standard sera, enzyme‑labeled secondary antibody, substrate, and stop solution. The manual details reagent preparation, sample dilution (1:101), incubation steps, washing procedures, and result calculation. It also specifies storage conditions (2‑8 °C, protected from light), a one‑year shelf life, and required equipment such as a plate reader with 450 nm/620 nm wavelengths. This manual is written in Chinese.
| Detection range | 7–150 U/mL |
|---|---|
| Storage temperature | 2‑8 °C |
| Shelf life | 1 year |
| Sample dilution | 1:101 |
| Applicable instrument | ELISA plate reader (450 nm, optional 620 nm) |
The kit can detect BP180 antibodies in the range of 7 U/mL to 150 U/mL.
Fresh serum should be used; if stored, it can be frozen at –20 °C for up to one month or at –70 °C for long‑term storage.
A microplate reader capable of measuring absorbance at 450 nm (and optionally 620 nm) is required.
Each patient serum should be diluted 1:101 (10 µL serum with 1 mL dilution buffer).