This document provides instructions for using the Chicken Growth Hormone (GH) Enzyme-Linked Immunosorbent Assay (ELISA) Kit, including information on the kit's composition, experimental principle, operating procedures, and sample requirements.
The kit is designed to determine chicken growth hormone (GH) concentrations in serum, plasma, or related liquid samples using a double‑antibody sandwich ELISA. The assay employs a GH‑coated microplate, a HRP‑labeled GH antibody, and TMB substrate, with absorbance read at 450 nm.
The manual details the kit components, sample handling requirements, step‑by‑step procedures for standard preparation, sample addition, incubation, washing, enzyme reaction, color development, termination, and data analysis, as well as safety and storage guidelines. This manual is written in Chinese.
| Measurement wavelength | 450 nm |
|---|---|
| Sample dilution factor | 5‑fold |
Absorbance should be read at 450 nm.
Standard samples are diluted stepwise as described, ending with a 5‑fold dilution of the final standard.