This kit uses the double antibody sandwich ELISA method to quantitatively determine the ECF content in rat serum, plasma or other related biological fluids.
This manual describes the rat Eosinophil Chemoattractant Factor (ECF) ELISA kit (model E0025Ra). It lists all supplied components, such as 96‑well plates, standards, detection reagents, and required equipment, and explains how to collect and store serum, plasma, or other biological samples for the assay.
The document details the assay workflow, including plate coating, sample addition, incubation at 37 °C, washing steps, substrate development, and absorbance reading at 450 nm. It also covers preparation of standard curves, data calculation, reagent storage at -20 °C, and safety warnings. This manual is written in Chinese.
| Detection range | 7.8 pg/mL – 500 pg/mL |
|---|---|
| Minimum detection limit | 3.4 pg/mL |
| Read wavelength | 450 nm |
| Incubation temperature | 37 °C |
| Incubation time | 2 h (first incubation) / 1 h (second incubation) |
The assay can quantify ECF concentrations from 7.8 pg/mL up to 500 pg/mL.
Read the plate at 450 nm after substrate development.
Incubate the plate at 37 °C for 2 hours during the first step and 1 hour after adding the stop solution.