About this manual
This manual provides detailed guidance for the rat Azidothymidine (AZT) ELISA immunoassay kit. It covers kit components, storage conditions, sample collection, reagent preparation, and the complete assay workflow from plate loading to result calculation.
Additional sections describe plate washing methods, precautions to avoid false positives, and tips for accurate optical density measurement at 450 nm. The document also includes calculation formulas and recommended practices for reliable quantitative results. This manual is written in Chinese.
What's inside
- Kit composition and reagent preparation
- Sample collection and storage
- Assay procedure
- Plate washing methods
- Data calculation
- Precautions
Specifications
| Shelf life | 6 months |
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| Long‑term storage temperature | -20 °C |
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| Assay plate format | 96‑well |
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| Standard stock concentration | 1000 ng/mL |
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| Reading wavelength | 450 nm |
Frequently asked questions
How long is the kit valid after manufacture?
The kit has a shelf life of six months.
Which wavelength should be used to read the assay results?
Measure the optical density at 450 nm.
How many wells does the assay plate contain?
The assay plate is a 96‑well plate.