The document introduces an automated sample preparation platform for online LC-MS/MS bioanalysis, which is used for pipetting liquid sampling. The platform offers fully automated sample processing of bio-fluids, including whole blood samples. It integrates the workflow from sample collection until the final analysis result, simplifying sample logistics and avoiding sample mix-up. The platform requires a minimum amount of sample (typically 5 µl) for quantitative results. It minimizes infection and contamination risk by using completely sealed and disposable sample containers, eliminating the need for re-used syringes or sample loops. The platform simplifies method development and allows for the use of the same generic method for different matrices. The fully automated process saves time and labor costs compared to traditional sample preparation, and also reduces costs for SPE materials, solvents, and other consumables. The SCAP PLS system components are mounted on a robotic platform.
The SCAP PLS system is an automated sample‑preparation platform that integrates sample collection, ultrasonic processing, and online LC‑MS/MS analysis. It uses disposable cartridges that serve as sealed sample loops, eliminating manual pretreatment steps and reducing contamination risk.
The system includes a robotic gripper, valve module with configurable single, dual, or triple valve options, and an ultrasonic module for blood cell disintegration. It can be interfaced with any leading mass spectrometer and is supplied with control software that manages valve switching and auxiliary hardware.
| Minimum sample volume | 5 µL (typical) |
|---|---|
| Maximum pressure (single valve) | 2 500 psi (170 bar) |
| Maximum pressure (dual/triple valve) | 20 000 psi |
| Valve drives per module | Up to 3 |
| Tip box capacity | 54 tips |
The system typically requires a minimum sample volume of 5 µL.
It is limited to a system pressure of 2 500 psi (170 bar).
The valve module can contain up to three valve drives.
Each tip box contains 54 numbered tips and caps.