This document describes the features and instructions for using the Rat Neuropeptide Y (NP-Y) Enzyme-Linked Immunosorbent Assay (ELISA) kit, including the detection range, minimum detection limit, specificity, shelf life, and anticipated applications.
This manual provides detailed instructions for the rat Nerve Peptide Y (NP‑Y) ELISA immunoassay kit. It describes the kit components, storage conditions, and preparation of reagents and standards, as well as guidelines for sample collection and handling.
Step‑by‑step assay procedures are outlined, including plate loading, incubation times, washing, substrate development, and termination. The document also explains how to generate a standard curve, calculate sample concentrations, and includes practical tips to ensure reliable results. This manual is written in Chinese.
| Detection range | 31.2 pg/ml – 2000 pg/ml |
|---|---|
| Minimum detection limit | 7.8 pg/ml |
| Specificity | No cross‑reaction with other related proteins |
| Shelf life | 6 months |
| Recommended storage temperature | -20 °C (long term) or 2‑8 °C (frequent use) |
| Assay plate type | 96‑well plate |
The kit can quantify NP‑Y from 31.2 pg/ml up to 2000 pg/ml.
The lowest detection limit is 7.8 pg/ml.
Store the kit at –20 °C for long‑term storage or at 2‑8 °C when used frequently.