Luminata TM Classico Western HRP Substrate is a premixed, ready-to-use reagent for chemiluminescent detection in western blotting applications that employ horseradish peroxidase (HRP)- conjugated antibodies. Luminata Classico Substrate is ideal for the detection of high to medium abundance proteins. It can be used with traditional immunodetection techniques or the SNAP i.d.® Protein Detection System. Visualization of target proteins is achieved through x-ray film or another imaging method.
Luminata Classico Western HRP Substrate is a premixed, ready‑to‑use chemiluminescent reagent for detecting HRP‑conjugated antibodies in western blot applications. It is suited for high to medium abundance proteins and can be visualized on x‑ray film or other imaging methods.
The manual covers package contents, storage (2‑8 °C, no light protection needed), detailed usage guidelines, recommended substrate volumes (≈0.1 mL per cm² of membrane), exposure times (1‑5 min), troubleshooting tips for high background or weak signal, and ordering information with catalogue numbers.
| Package volume (100 mL) | 100 mL |
|---|---|
| Package volume (500 mL) | 500 mL |
| Storage temperature | 2‑8 °C |
| Membrane coverage (low volume) | 1,000 cm² |
| Membrane coverage (high volume) | 5,000 cm² |
| Substrate required per cm² | ≈0.1 mL |
| Typical exposure time | 1‑5 minutes |
Store it at 2‑8 °C; protection from light is not required.
Approximately 0.1 mL of HRP substrate is required for each cm² of membrane.
Typical exposure time is 1 to 5 minutes, depending on the antibody system.